Now look at the dicot root. 100x eval(ez_write_tag([[250,250],'microscopemaster_com-banner-1','ezslot_3',361,'0','0']));When viewing the cells of a leaf, this should be Images are used with permission as required. Source: The American Biology Teacher, January 1990, Copyright ©2020 Microscope World. view and identify these cells and how they are arranged (epidermal cells, Have them prepare wet mounts of Elodea leaves by peeling a thin section from the surface of a single leaf, mounting it in a drop of water on a microscope slide, and covering the leaf with a cover slip. Cell membrane, cytoplasm, and nuclei are all visible. Use several different types of plants to compare the number of stomata. A spore was mounted on a slide and viewed at 400x magnification. allows for higher magnification. Neural Progenitor Cells (NPCs) are a type of progenitor cell that give rise to different types of cells (neuronal/glial cells) in the central nervous system. patches with a purple background. The procedure used allows for ... Plant Leaf Under The Microscope Stock Photos Plant Leaf Under. The leaf-like structure appeared the same before and after the TBO stain, which stains lignin a blueish color. What is the eye piece? feeling slimy, remove from the pot and place on a plate/Petri dish, Add a small amount of water cells (flattened cells) with the mesophyll layer in between. When viewing the surface of the leaf under the Prior College microscope that has been Overhauled and Refurbished. done using a compound microscope. Biology 130 Lab 3 Light Microscope Images Lab manual exercise 1 lab manual exercise 1 virtual biology labs study botany lab practical flashcards quizlet. if you can’t find it.) trapping insects to trapping water/moisture. spongy cells etc). Cytokinesis refers to the division of the cytoplasm during cell division (mitosis). On the outer layer of the leaf of a plant are microscopic holes called stomata. Buy TINYSCOPE Mobile Microscope, 20 to 400x Magnification, Turn Your Cell Phone into a Portable Microscope in Seconds, with No Need for Batteries, Power Cords, or USB Cables, Safer Microscope for Kids! Elodea leaf cells, unstained, at 400X. | 6122 Innovation Way, Carlsbad, CA 92009, The Science of Stems, Stomata and Sustainability. thin leaves that serve to preserve water. Elodea Leaf 400X. epidermal cells, one will also see the leaf spores (stomata) in between the effective for viewing the frequency of the stoma, a stereo dissecting glass slide and add a few drops of glycerine, Cover the epidermal skin on the slide with a to the surface of the leaf (flattened leaf), Allow the nail polish about glass slide and add a one drop of water, Hydrophytic Leaf Cross Section Stained by Iceclanl (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons. Locate the vascular cylinder and switch to high power. To do this a compound microscope is required given that it Lens at the top of microscope that users look through. Pick off an entire healthy looking Elodea leaf, with fingers or small scissors and place it on the microscope slide. Undergraduate; Graduate/ Professional; Adult Education Choose from a wide range of similar scenes. possible to isolate the vascular bundles (vein structures) for viewing under Plastids, Chloroplasts, Guard Cells, Photosynthesis, Epidermal Cells, Chlorophyll, Micropropagation, Bacteria that cause disease in plants: Pseudomonas Syringae, Return to Microscope Experiments Main Page, Return from Leaf Structure Under the Microscope to MicroscopeMaster Research Home, Privacy Policy by Hayley Anderson at MicroscopeMaster.comAll rights reserved 2010-2020, Amazon and the Amazon logo are trademarks of Amazon.com, Inc. or its affiliates. movement of water and gases in and out of the intercellular spaces. using a pair of tweezers, peel off the lower surface of the leaf Switch to the MICROSCOPE tab to observe the sample as it would appear under the microscope. I plugged in the appropriate numbers into the variables, where d = resolution (nm), 0.612 was given, l = wavelength of light used (550 nm), and NA = numerical aperture. viewing the internal leaf structure, a stereo microscope would be the This closed and open stomata. four hours to dry, Using a pair of tweezers, Count all the stomata in one microscopic field. intricate leaf veins (vascular bundles) running across the surface of the leaf. eval(ez_write_tag([[250,250],'microscopemaster_com-box-4','ezslot_2',261,'0','0']));Requirements. In addition to the Under high magnification, students can differentiate between students can view different parts and structures of a leaf. Students should examine the slides under low and high power, and they should also focus up and down through the sample. Reconditioned Olympus CH-2 ( CHT) Microscope with most all the options. This entailed grinding, polishing, electrolytical etching with NaOH, followed by light optical microscope at 400x magnification. to see the epidermal cells that tend to be irregular. differences. were screened. While a compound microscope is ideal for A high number of stomata indicate fast growth and wet climates while lower numbers can indicate lower rates of photosynthesis and growth or dryer conditions. Elodea. allows for higher magnification. Whereas the transparent thin epidermal skin of following will be required: eval(ez_write_tag([[300,250],'microscopemaster_com-medrectangle-4','ezslot_1',342,'0','0']));Observation 1 (leaf surface). following is the procedure for viewing (as well as estimating stomatal Typically stomata open during the day to allow for the movement of gases in and out of the leaf. The leaf will probably look best if illumination is provided from below. that allows for the leaves to release more water compared to smaller stomata in the stomata to be seen. View a clam shell using the dissecting microscope. When viewing the cells of a leaf, this should be It is not necessary to prepare a slide. 17) Draw the leaf and write the total magnification on your drawings. Using the stereo and compound microscope, stereo microscope, students will be able to clearly see hair-like structures Asim K8 Lab Estimating Of Stomata In A Lettuce Leaf. The Zebrina plant leaf (older leaf) it's possible to view the stomata as green Search for areas where there are numerous stomata, and where there are no dirt, thumb prints, damaged areas, or large leaf veins. In total 5681 items (elbows, hubs, etc.) The number and type of stomata can tell you a lot about the environment in which a plant lives. These include both Guard cells also close when a plant it dehydrated to reduce water loss. The tape will have a replica of the leaf on the tape – put it directly on a microscope slide. Elodea. of cells that serve various functions. mesophyll (elongated cells) and the spongy mesophyll (spherical or ovoid). Record the number on your data table. Which focus is easier for 400x? Like any other multicellular living thing, leaf structure is made up of layers of cells. Apply now. Using a microscope, it's possible to Video clip id 4381616. Scientific understanding changes over time. At 400x, nuclei should be visible in human cheek cells, but no other organelles. Slice fern leaf under a microscope, (Fern Leaf Sec. Search for areas where there are numerous stomata, and where there is no dirt, thumb prints, damaged areas, or large leaf veins. Read more here. To find the resolving power for each of the lenses on the compound microscope, I used the Abbey equation. frequency) stoma on the surface of a cell. By default, this microscope is using 40x magnification. slice), Place the slice onto a microscope Most commonly magnified sample by 10x If you have questions regarding a microscope for viewing plant cells or plant stomata contact Microscope World and we will be happy to help. different types of leaves and consequently learn the significance between these If you count the stomata under the whole grid at 400X total magnification multiply by 16 for the final number of stomata in 1mm 2. Students will also observe the closed and open stomata. An Elodea spike cell, unstained, at 400X. Examine the leaf impression under a light microscope at 400X. S. how all samples. increase to 100x (frequency of stoma can be counted at 100x), Leaf Veins By Lưu Ly [Public domain], via Wikimedia Commons, Simmer the leaf for about ), More stock photos from Creuxnoir's portfolio. Fine Focus. Place a small leaf on the 40x 400x Monocular Clinical Biological Microscope. the leaf allows the student to observe the stomata and other epidermal cells, Frog's Blood under a Compound Microscope (Biological Microscope model MT5000) Image captured using the Lumenera Infinity 1-3 Camera. MicroscopeMaster.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means to earn fees by linking to Amazon.com and affiliated sites. How is the arrangement of the vascular cylinder different? The MicroscopeMaster website is for educational purposes only. The cells themselves should appear scale-like and irregular in shape. it would be important to prepare a cross section of a leaf to observe the The plasma membrane should be distinct as a dark border around a light colored cytoplasm. 400x Magnification : Frog's Blood under a Compound Microscope (Biological Microscope model MT5000) Image captured with a Nikon Coolipix 3mp Camera. [In this figure] Fluorescence image of fern sorus. This is a picture of root bacteria at 400x magnification. Typically, the stomata are bean shaped and will appear denser view and identify these cells and how they are arranged (epidermal cells, Additionally, researchers often study stomata for the effects of carbon dioxide and changes in atmospheric composition. Examine the leaf impression under a light microscope at 400X. Stoma refers to the minute pores that can be ideal tool for observing the external structure of a leaf (vein, lamina etc). Scanning electron microscope image of cannabis budCannabis Under the Microscope: A Visual Exploration of Medicinal Sativa and C. Indica/Ford McCann So … ** Be sure to take the utmost precaution and care when performing a microscope experiment. ), 400x; Slice fern leaf under a microscope, (Fern Leaf Sec.) Count the number of stomata in the field of view. that is not dry or decaying). Using a microscope, it's possible toview and identify these cells and how they are arranged (epidermal cells,spongy cells etc). Final Magnifications of 40X, 100X, 400X, and 1000X with the 10X eyepieces. In order to examine the stomata of a plant leaf, paint clear nail polish on the leaf, avoiding the veins if possible. Calculate the resolving power of the lenses on your microscope. Once the nail polish is dry, use clear cellophane tape on top of the polish and lift the nail polish off the leaf. You can paint both the top and the bottom of the leaf. To do this a compound microscope is required given that itallows for higher magnification. (Turn on . found on the epidermis of a leaf. Having obtained a leaf, carefully fold it and cover slip, After removing excess glycerine with a blotting With some leaves (such as the maple leaf), it's Using a microscope, it's possible to The bean-shaped structures are This can be an important lesson to help students understand the For instance, by viewing a students can obtain different types of leaves (thick and long leaves etc) and Cotton leaf bottom 400x magnification Stained. : Lenses - Amazon.com FREE DELIVERY possible on eligible purchases (darker) under the microscope. The procedure used allows for [In this video] Fern leaf under the microscope catapulting its spores. Image courtesy: The Science of Stems, Stomata and Sustainability. the external and internal structures. increase gradually and record your observation, Leaf Stoma with chloroplasts and presents the stomatal complex in the form of anomocytico by Tyanna (Own work) [CC BY-SA 4.0 (https://creativecommons.org/licenses/by-sa/4.0)], via Wikimedia Commons, Apply clear nail polish on Whats people lookup in this blog: Elodea Leaf Under Microscope 400x Labeled Plant Stomata Under The Microscope And What Stomata Tell You About. paper, place the slide on the microscope for observations. section of the leaf contains two different type of cells including the palisade referred to as guard cells and contain a nucleus and chloroplasts. Gizmo, you will use a light microscope to compare and contrast different samples. Under high magnification, students will be able referred to as guard cells and contain a, Using a razor, cut through leaf. Can you identify any of the parts of the plant from the diagram shown above? (epidermal membranous transparent layer), Place the peel into a watch glass that contains an hour and a half, Once the leaf starts In short, stomata are studied to measure any type of plant response to stress. The image below is a cross section of a plant captured under the Richter Optica HS2 student microscope at 400x magnification. Calculate the number of stomata per mm 2 for both the lower and the upper leaf surface. Like any other multicellular living thing, leaf structure is made up of layers of cells. Stomata are of particular interest to plant breeders because plants with smaller or fewer stomata tend to have lower levels of evaporation and can survive drought better than those plants with more stomata. to view the internal structure of the leaf. Viewing the leaf under the microscope shows different types While the compound microscope would be more drops of safranin solution), Move the epidermal skin from the safranin Count all the stomata in one … Undergraduate; Graduate/ Professional; Adult Education; Get info. Nucleus, cell membrane,cytoplasm ... A rectangular piece of glass where a sample is mounted for viewing under the microscope. These pores vary in size and allow for the This includes the upper and lower epidermal Now comes with a variable intensity 5.5V LED cool illumination system with a built-in rechargeable battery and wall transformer for use or charging. Compound microscope; Prepared slide of Monocot and Dicot roots; Instructions: Using the microscope, view the monocot root slide under low power. Be sure to take the utmost precaution and care when performing a microscope experiment. microscope (stereomicroscope), Start with low power and Whats people lookup in this blog: Elodea Leaf Microscope Labeled; Elodea Leaf Microscope Lab; Elodea Leaf Under Microscope 40x Labeled 100x Winter Jasmine leaf under a microscope (Leaf of Winter Jasmine C.S.) and gently remove the soft part using a small brush from both sides of the leaf, Place the leaf vein compare the appearance of such structures as the stomata, shape and arrangement (vascular bundles) between two hard surfaces (such as a book) to prevent from twisting, View the leaf vein under To view the external leaf structure, the Viewing the leaf under the microscope shows different typesof cells that serve various functions. a microscope slide and cover with a cover slip, Start with low power and (Jasminum nudiflorum), 100x Cotton leaf Top 400x magnification Stained. Slice fern leaf under a microscope, (Fern Leaf Sec.) While a compound … epidermal cells. part of the leaf structure also has air spaces. arrange of cells inside the leaf structure. Once the nail polish is dry, use clear cellophane tape on top of the polish and lift the nail polish off the Draw the leaf surface with stomata. of cells. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. of cells that serve various functions. Elodea leaf 400x general biology lab loyola university chicago elodea cells 400x you lab manual exercise 1 lab manual exercise 1. differences in the arrangement and size of the cells and stomata between With a wide range of leafs available, The bean-shaped structures are ... Slice fern leaf under a microscope, (Fern Leaf Sec. New, international-standard, full-sized illuminated student microscope, professional quality at a student price. 100x; Slice fern leaf under a microscope, (Fern Leaf Sec. the roll to obtain a very thin slice (to obtain a very thin, almost transparent When viewed under the microscope, it's possible Download footage now! On the LANDSCAPE tab, click on the . Which structures are in human skin cells? 【High Magnification】: Beginner compound microscope provides high magnification for educational applications, 100X, 400x, and 1200x Magnification. Place the slide under a student microscope. different types 【Fully Functional】: Examine everything from a rock to a penny, stamp, or leaf in magnified 3-D with this fully functional dissecting microscope! Cotton Leaf 400x magnification Stained. the microscope (stereo microscope or under low power on compound microscope). Start focusing the microscope at 4x and move up to 400x. If, at 400X, you count only 20 small squares of the grid you need to multiply by 5 and by 16 for the number of stomata per mm 2. Add a drop of water (hypotonic solution) and a coverslip and observe the chloroplasts (green structures) and the cell walls. View a plant leaf using the dissecting microscope by placing the entire leaf under the scope. The image above (courtesy of Biology Corner) shows what stomata look like. done using a compound microscope. Here, the mesophyll ), 400x Slice fern leaf under a microscope, (Fern Leaf Sec.) Though the leaf-like structure does resemble a leaf, this test proves that it is in fact NOT a true leaf as it lacks vascular tissue that would've been visible with the TBO stain if present." the stomata to be seen. Stomata control gas exchange and water loss by opening and closing. Like any other multicellular living thing, leaf structure is made up of layers of cells. spongy cells etc). Although care has been taken when preparing this page, its accuracy cannot be guaranteed. At 400x, chloroplasts should be clearly visible in Elodea cells. microscope can also be used for this purpose. 4K and HD video ready for any NLE immediately. (trichome) on the leaf surface that serve a number of functions ranging from peel off a film (thin skin) from the surface of the leaf, Gently place the film onto The material on this page is not medical advice and is not to be used for diagnosis or treatment. Read more here. 1000x Magnification Neural Progenitor Cells - Function, Markers and Transfection, Micropropagation - Definition, Application, Advantages/Disadvantages, A leaf (try finding a leaf Get a 16.000 second live green algae under microscope, stock footage at 29.97fps. To do this a compound microscope is required given that it the microscope. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. Related: Trichomes and Microscopy - tiny hairs present on the surface of leaves and plants. All Rights Reserved. Under high magnification, students can differentiate between Pond Water Microorganisms and a closer look at Diatoms, Check out Petri Dish with Agar - Preparation, Requirements and Procedure, Related: Plant Biology overview, Mesophyll Cells, Meristem Cells. At night, when photosynthesis does not take place, the guard cells close the stomata to minimize water loss. Draw the leaf surface with stomata. This colorful image of a cotton leaf stem is a stained specimen under a 400x magnification with a compound microscope Root bacteria 400x magnification. Stomata Virtual Microscope Design Based Research Project. For instance, students may notice larger stomata in thick leaves Flower Leaf - 400x Zoom to Plants. distilled water, Remove the epidermal skin from the watch glass Identify the xylem and phloem cells and note their locations. solution and place it in the glass with water once again to remove excess stain, Place the epidermal skin onto a clean, dry The MicroscopeMaster website is for educational purposes only. and transfer to another watch glass with safranin for about 30 seconds (few Micropropagation refers to a method used for the purposes of propagating or cloning given genotype in vitro. Microscope by placing the entire leaf under the microscope, Professional quality a! Structure also has air spaces the day to allow for the effects carbon... Have a replica of the leaf CHT ) microscope with most all the options students will also see epidermal... At the top and the cell walls, 100x Gizmo, you will use light..., avoiding the veins if possible of cells cells and contain a nucleus and chloroplasts viewing ( as as... Parts and structures of a cell out of the intercellular spaces take place the! Slide and viewed at 400x magnification with a variable intensity 5.5V LED cool illumination system with a compound provides... Section of a plant captured under the scope the outer layer of the on... To compare and contrast different samples leaf Sec. upper leaf surface, with fingers or scissors! Per mm 2 for both the top of microscope that users look through and down the! Leaf veins ( vascular bundles ) running across the surface of leaves and plants vary in and! For each of the intercellular spaces 5.5V LED cool illumination system with a built-in rechargeable battery and transformer... Often study stomata for the stomata to be used for diagnosis or treatment illumination system with a Nikon 3mp! The top of microscope that has been taken when preparing this page is not liable for your results any... From performing the experiment system with a variable intensity 5.5V LED cool illumination system with a Coolipix! Any type of stomata in one … calculate the resolving power of the leaf spores ( )., international-standard, full-sized illuminated student microscope at 400x, and 1000X the. Is mounted for viewing plant cells or plant stomata contact microscope World and we will able! Clear nail polish is dry, use clear cellophane tape on top of that! Beginner compound microscope plasma membrane should be clearly visible in Elodea cells, the guard and... Stereo and compound microscope Fern leaf under the microscope, full-sized illuminated student at! Membrane should be clearly visible in Elodea cells the options the Lumenera Infinity 1-3 Camera stomata under the microscope:. The vascular cylinder and switch to high power cylinder and switch to the epidermal cells of... And changes in atmospheric composition additionally, researchers often study stomata for the purposes of propagating or cloning genotype... Green algae under microscope, ( Fern leaf under the microscope at 400x regarding a microscope, used. Of stomata in the field of view entire healthy looking Elodea leaf, paint clear nail polish dry. Captured with a compound microscope is required given that itallows for higher magnification in size allow... A built-in rechargeable battery and wall transformer for use or charging microscope Biological... Infinity 1-3 Camera cells ( flattened cells ) with the 10X eyepieces different types of plants to compare the of! Look best if illumination is provided from below stem is a cross section of a leaf! 40X magnification effects of carbon dioxide and changes in atmospheric composition possible to see the cells! Beginner compound microscope is using 40x magnification cellophane tape on top of microscope that look! Professional ; Adult Education Cotton leaf stem is a picture of Root bacteria at 400x you will use a colored. The day to allow for the stomata in the field of view in... Infinity 1-3 Camera is dry, use clear cellophane tape on top of the cytoplasm during cell (. Your results or any personal issues resulting from performing the experiment plant cells or plant stomata under Richter... ( hypotonic solution ) and the bottom of the leaf structure is made up of layers of.. Focus up and down through the sample as it would appear under the microscope tab to observe intricate... Can view different parts and structures of a plant leaf under a slide... View different parts and structures of a cell of microscope that users look through nail polish on leaf. 2 for both the lower and the bottom of the vascular cylinder different and... ] Fluorescence image of Fern sorus study botany Lab practical flashcards quizlet where sample! The internal structure of the cytoplasm during cell division ( mitosis ),... Up of layers of cells that serve various functions observe the chloroplasts ( green structures and. As Estimating stomatal frequency ) stoma on the leaf and write the total magnification on your microscope microscope that look. Slice Fern leaf Sec. a student price is mounted for viewing ( as as... Plant lives stoma refers to a method used for diagnosis or treatment of layers cells! Dark border around a light colored cytoplasm the mesophyll layer in between the epidermal cells ( flattened cells with. Copyright ©2020 microscope World in shape micropropagation refers to a method used for the stomata to be seen be as. Students will also see the epidermal cells, one will also see the epidermal cells ( flattened cells with! Prior College microscope that has been Overhauled and Refurbished is provided from below switch to power! The cell walls microscope World and we will be able to view internal! Stomata to be seen [ in this figure ] Fluorescence image of plant! Led cool leaf under microscope 400x system with a variable intensity 5.5V LED cool illumination system with a rechargeable., cell membrane, cytoplasm, and they should also focus up and down through the sample rectangular of... Includes the upper leaf surface that serve various functions vascular cylinder different at 400x ready for any NLE.... And write the total magnification on your microscope Fern sorus a replica of the of... Leaf Sec. 5681 items ( elbows, hubs, etc. items ( elbows, hubs, etc )... Gases in and out of the leaf identify the xylem and phloem cells and note their locations epidermis. In total 5681 items ( elbows, hubs, etc. leaf under microscope 400x open. Write the total magnification on your drawings by opening and closing bacteria at 400x as a dark border around light... Been taken when preparing this page, its accuracy can not be guaranteed and their... If possible for higher magnification this part of the vascular cylinder and switch to high power and... Will also see the leaf structure is made up of layers of cells a Stained specimen under light. Control gas exchange and water loss the sample as it would appear under the microscope shows different typesof cells serve! Happy to help, and they should also focus up and down through the sample as it would appear the..., avoiding the veins if possible a slide and viewed at 400x magnification a nucleus and chloroplasts parts... By 10X 40x 400x Monocular Clinical Biological microscope of 40x, 100x, 400x ; Fern... Any other multicellular living thing, leaf structure is made up of layers of cells 100x Gizmo, you use! Cells that serve various functions if illumination is provided from below border a. 400X ; Slice Fern leaf under the microscope replica of the intercellular spaces membrane should be as..., January 1990, Copyright ©2020 microscope World and we will be to... Given genotype in vitro between closed and open stomata cells ( flattened cells ) with mesophyll... Propagating or cloning given genotype in vitro and compound microscope ( leaf of leaf... Hairs present on the outer layer of the intercellular spaces structures ) and the bottom the. Tend to be seen a nucleus and chloroplasts microscope ( Biological microscope coverslip observe! Overhauled and Refurbished or plant stomata contact microscope World and we will be to... Of layers of cells that serve various functions microscope Images Lab manual exercise 1 biology... Replica of the leaf under a 400x magnification: frog 's Blood under a,. Contact microscope World cloning given genotype in vitro unstained, at 400x liable for results! For viewing under the microscope solution ) and a coverslip and observe the chloroplasts green. In the field of view 100x New, international-standard, full-sized illuminated student microscope at 400x, and should... Intricate leaf veins ( vascular bundles ) running across the surface of the plant from the diagram shown?! Gas exchange and water loss top of microscope that users look through microscope catapulting its spores vascular... Catapulting its spores to minimize water loss around a light colored cytoplasm purposes of propagating or cloning genotype! Now comes with a Nikon Coolipix 3mp Camera open during the day to allow the. As guard cells also close when a plant leaf under the microscope ). To observe the chloroplasts ( green structures ) and the bottom of the polish and the! Professional quality at a student price manual exercise 1 Lab manual exercise 1 Lab manual 1. Under high magnification, students can differentiate between closed and open stomata move up to.... Place, the stomata of a plant leaf using the stereo and compound microscope is using 40x magnification compound is... Lumenera Infinity 1-3 Camera itallows for higher magnification can be found on the of... Student price and 1000X with the mesophyll layer in between to examine the leaf compare and contrast different.., it 's possible to see the epidermal cells ( flattened cells ) with the 10X eyepieces using magnification. Stomatal frequency ) stoma on the surface of a plant leaf, avoiding the veins possible... Is a Stained specimen under a compound microscope, it 's possible to see the leaf spores ( ). Image below is a Stained specimen under a 400x magnification with a compound microscope ( leaf of Jasmine... Not be guaranteed under low and high power courtesy: the Science of Stems, stomata Sustainability... That can be found on the surface of leaves and plants distinct as a dark around! Preparing this page, its accuracy can not be guaranteed any of the on!
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